*Thermofisher Scientific 89818

Thermo Scientific Pierce Biotin 3'末端DNA标记试剂盒用于标记带有生物素的单链DNA引物，用于非放射性电泳迁移率变动分析（EMSA）和其他核酸检测方法。

DNA生物素化程序使用末端脱氧核苷酸转移酶（TdT）来催化非模板指导的核苷酸掺入单链DNA的3'-OH末端。 TdT表现出对单链DNA的底物偏好，但它将用3'突出端和平端双链体标记双链DNA，尽管效率较低。

生物素3'末端DNA标记试剂盒的特点：

•非同位素标记消除了危险放射性物质或难以处置的废物的麻烦
•1-3个生物素化的核糖核苷酸到DNA链的3'末端，对蛋白质的杂交或序列特异性结合的干扰较小
•生物素标记的探针稳定一年以上
•30分钟的标签程序快速有效

生物素3'末端DNA标记试剂盒已经过优化，可将1-3个生物素化的核糖核苷酸（生物素-11-UTP）整合到DNA链的3'末端。该标记策略具有将生物素定位于探针的3'末端的优点，其中它不太可能干扰蛋白质的杂交或序列特异性结合。生物素标记的DNA探针可用于促进多种应用中的非同位素检测，包括电泳迁移率变动分析（EMSA），Northern或Southern印迹，菌落杂交或原位杂交。

*Thermofisher Scientific 89818

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.