thermo二抗A21207|Invitrogen抗体*

我们提供广泛的Invitrogen™二抗结合物，具有良好的特异性特征，并使用多种优质荧光染料进行标记，包括Invitrogen™Alexa Fluor™荧光染料。 荧光二抗缀合物可用于其特定靶标的检测，分选或纯化，是荧光显微镜和共聚焦激光扫描显微镜，流式细胞仪和荧光西方检测的理想选择。 我们提供的荧光标记的广泛性使我们的试剂可以适应几乎任何荧光检测系统。

可以以三种形式提供二抗：完整IgG，二价F（ab'）2片段和单价Fab片段。 由于许多免疫球蛋白结构域的结构具有高度保守性，因此大多数类特异性第二抗体必须经亲和纯化和交叉吸附以实现与其他免疫球蛋白的小交叉反应。

我们的二抗结合物较常通过用来自目标物种的汇集的免疫球蛋白群免疫宿主动物来制备，并且可以进一步纯化和修饰（例如，免疫亲和层析，抗体片段化，标记缀合等）以产生高度特异性的试剂。。 在*轮纯化中，回收与免疫抗体结合的完整免疫球蛋白，并且主要由~150-kDa IgG类组成。 进一步纯化，例如，用蛋白A或G，除去与靶特异性免疫球蛋白重链和/或轻链反应的亲和纯化的抗体，除去所有不需要的免疫球蛋白类别。

thermo二抗A21207|Invitrogen抗体*

Product Specific Information

To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor染料是目前较值得信赖的荧光染料之一。 Invitrogen™Alexa Fluor 594染料是一种明亮的红色荧光染料，具有理想的激发，适用于594 nm激光线。为了在成像和流式细胞仪中产生稳定的信号，Alexa Fluor 594染料在很宽的摩尔范围内对pH不敏感。具有高荧光量子产率和高光稳定性的探针允许以高灵敏度检测低丰度生物结构。 Alexa Fluor 594染料分子可以高摩尔比附着在蛋白质上而不会发生明显的自猝灭，从而实现更明亮的结合物和更灵敏的检测。每种缀合物的标记程度通常为每个IgG分子2-8个荧光团分子;每个产品批次的分析证书上都标明了确切的标签程度。

使用结合物溶液：使用前在微量离心机中短暂离心蛋白质结合物溶液;仅将上清液添加到实验中。该步骤将有助于消除在储存期间可能形成的任何蛋白质聚集体，从而减少非特异性背景染色。由于染色方案随应用而变化，因此应根据经验确定抗体的适当稀释度。对于荧光团标记的抗体，对于大多数免疫组织化学和流式细胞术应用，终浓度1-10μg/ mL应该是令人满意的。

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-10 µg/mL

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 6 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 6 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 5 publications below

Immunocytochemistry (ICC)

See 2 publications below

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Miscellaneous PubMed (MISC)

See 13 publications below

Product Specifications

Species Reactivity

Rabbit

Host / Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 594

Excitation/Emission Profile

View spectra 

Form

liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_141637

Target

IgG

Antibody Form

Whole Antibody